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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Nikon A1R with PicoQuant FCS/FLIM in Ti-E (Inverted).
Techniques
1. Four colour confocal microscopy
2. Fluorescent Lifetime Imaging (FLIM)
3. Fluorescence Correlation Spectroscopy (FCS)
4. Multipoint and live time-lapse imaging
5. High speed resonant scanning
6. JOBS multidimensional acquisition software
Instrument specifications
1. Inverted Nikon TiE microscope
2. Nikon PlanApo objectives 10×- 40×, air and oil immersion
3. Nikon PlanApo 60× WI IR, 1.27NA objective
4. Nikon PlanApo 100× Oil immersion, 1.45NA objective
5. Four 50mW solid state lasers - 405 nm (violet), 488 nm (blue), 561 (yellow), and 640 nm (red)
6. Two PMT detectors
7. Two Gallium Arsenide Phosphatide (GaAsP) detectors
8. Galvanometer and resonant scanners
9. Three picosecond pulsed lasers - 405 nm (violet), 485 nm (blue) and 640 nm (red)
10. Picoquant Symphotime 64 analysis software
11. Two PMA hybrid detectors
12. Piezo stage
13. Perfect Focus System III
14. NIS Elements software controlling confocal acquisition and lasers
15. Oko Labs stage top incubator
This inverted confocal has Picoquant module attached to enable Fluorescent Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) imaging. FLIM is a technique which investigates the exponential decay rate, or lifetime, of a fluorophore, which is sensitive to changes in the local environment. FLIM is also used to interrogate FRET pairs, providing a stronger correlation than analysis by confocal means. FCS enables the investigation of single molecule diffusion dymanics within a femtolitre volume.
This system comprises of four solid state lasers with two photomultiplier fluorescence detectors and two Galllium Arsenide Phosphatide (GaAsP) detectors for confocal imaging, as well as three picosecond pulsed lasers (405 nm, 485 nm and 640 nm), with two PMA hybrid detectors. Furthermore, there are both galvanometer and resonant scanners on this platform to facilitate various imaging speed requirements. FLIM and FCS images are acquired using PicoQuant Symphotime.
Live cell ima
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.