Optical Microscope Imaging Service—Axiovert Z1

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Axiovert Z1.

Name of the Instrument/Platform

Axiovert Z1

Introduction

1. Imaging: Brightfield + Pase Contrast + Fluorescence
2. Detectors: AxioCam 208 - 8 Mpx 4k CMOS color camera dedicated to Brightfield imaging.
3. Stand:
1) Inverted
2) Zeiss Axiovert Z1
4. Stage: Manual in XYZ
5. Software: Zeiss Zen Blue Lite for camera control
6. Illumination:
1) White light for Brightfield and Phase contrast (transmitted light)
2) Blue/Green/Red LED for fluorescence
7. Filter cubes:
1) 01 BFP (DAPI, blue)
2) 38 GFP (green)
3) 43 RFP (Cy3, red)
8. Contrast: Brightfield, Phase contrast
• Objectives
1. Objective: N- AchroPlan, N- AchroPlan, LD-PN, Plan Apo, LD-PN
2. Mag. / NA / Phase: 5× / 0.15 / Ph1, 10× / 0.25 / Ph1, 20× / 0.4 / Ph2, 20× / 0.8, 40× / 0.6 / Ph2
3. Medium: Air, Air, Air, Air, Air

Applications

1. Brighfield imaging: H&E, Sirius red, etc.
2. Phase contrast: Prokaryotic or Eukaryotic unlabeled cells grown on vessel.
3. Widefield microscopy: Metal halide lamp and filter-based microscope for checking green/red stainings.

Summary

This basic, inverted Zeiss Axiovert Z1 microscope allows you to check your brightfield and fluorescent stainings by using either transmitted white light or LED-based fluorescence excitation (blue, green, red). Additionally, the system boasts phase contrast objectives, that can be used to introduce contrast into unstained samples. The microscope is fitted with an AxioCam 208 color camera, particularly suitable for brightfield imaging. This microscope is ideal if you want to take a few snapshots of your H&E or Sirius red staining, for example, or if you just want to check if your fluorescent staining worked. The phase contrast objectives are ideal to look at unlabeled Prokaryotic or Eukaryotic cells on culture vessels, since the microscope boasts an inverted stand. This microscope does not need to be booked when checking samples by eye, but please do if you will be taking images with it.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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