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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Zeiss LSM 510 META NLO.
Microscope: Zeiss Axio Imager. Z1
1. motorized 6 position filter cassette
2. motorized revolving nosepiece
3. 100 W halogen lamp as transmission light
4. microscope table with high-precision z-drive (increment (10 +/- 10) nm)
5. achromatic aplanatic universal condensor with 7 position turret containing
1) Pos. D: dark field diaphragm
2) Pos. H: none, for standard brightfield illumination
3) Pos. 1: phase stop for Ph1 objectives
4) Pos. I: none
5) Pos.2: phase stop for Ph2 objectives
6) Pos. II: DIC prism for DIC II sliders
7) Pos.3: phase stop for Ph3 objectives
8) Pos. III: DIC prism for DIC III sliders
Incubation Chamber
1. Direct sample heating via Heatable Universal Mounting Frame A-H from PeCon with temperature controller Tempcontrol 37-2 digital from PeCon
2. Sample and microscope heating via homemade opaque incubation chamber (see picture)
1) with large heating plates inside controlled via homemade temperature controller
2) equipped with CO2 supply and humidifier
3)
The laser scanning microscope 510 META NLO from Zeiss is based on the reflected light microscope Axio Imager. Z1. In addition to an Argon laser and two He-Ne laser, covering the spectrum of visible light for laser excitation, the setup contains a tunable titanium:sapphire laser of the Chameleon Ultra Family from Coherent for multiphoton imaging. The latter emits light from the red and the near infrared in the range of 690 nm to 1040 nm.
Fluorescence detection is accomplished via two photomultiplier tubes (PMT) and the spectral META detector. The latter is an array of 32 PMTs. Fluorescence light is directed onto a wavelength dispersive element and is imaged onto the META detector. Each PMT of the META detector covers a spectral range of 10.7 nm, resulting in a total spectral width of approx.340 nm (380 - 720 nm). The META detector is a powerful tool for spectral imaging and unmixing. Furthermore, a transmission detector (PMT) detects the scanning laser and provides a transmission light
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.