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The characterization of molecules is an important part of the process, enabling the physical and chemical properties of molecules to be resolved. CD BioSciences has excellent analytical staff, years of analytical experience, and cutting-edge instrumentation. Using LC-MS, multiple HPLC, and other techniques, we can accurately quantify/characterize the DAR of coupled drugs, residual small molecules, endotoxins, and more.
System components:
1. Wyatt DAWN HELEOS II-multi-angle (static) light scattering (MALS) detector. The molar mass and root mean square radius of the solute are determined by measuring the intensity of the scattered light-provided that the refractive index increment (dn/dc) of the solute/solvent system is known.
2. Wyatt Optilab T-rEx-refractometer for measuring the absolute refractive index and its increment
3. Wyatt QELS-quasi-elastic (dynamic) light scattering (QELS) detector. The diffusion coefficient/hydrodynamic radius can be determined from the temporal fluctuations in the intensity of scattered light
4. HPLC-with size-exclusion columns (SEC), DAD absorbance, and fluorescence detectors
In this set-up the sample components are separated according to size and characterized individually by static and dynamic light scattering, absorbance and fluorescence.
For proteins and nucleic acids: measurements of molecular weights and radii, oligomerization, heterogeneity and solubility studies, characterization of macromolecular complexes in terms of size, overall shape and composition/stoichiomentry.
For synthetic polymers: measurements of molar mass & mass moments, polydispersity, conformation/branching.
For nanoparticles: solubility and size distribution measurements and composition /stoichiometry analysis for functionalized nanoparticles.
The SEC-MALS-QELS system is composed of a size exclusion chromatography system (SEC) followed by a Wyatt DAWN HELEOS II multi-angle static light scattering detector, a Wyatt QELS dynamic light scattering detector, and a Wyatt T-rEx differential refractive index detector.
CD BioSciences offers its customers SEC-MALS-QELS-based molecular characterization services that contribute to the process and drug development process of biomolecules. If you need our technical support, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.