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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with In-Vivo 2-Photon Microscope-based confocal microscopy imaging services.
1. Observation of tissue slices or entire animals
2. Photomanipulation: uncaging, photo-activation, optogenetic, etc. ..
3. Possible coupling with electrophysiology for electrical stimulation or Ephys recording
• Designation
Microscope 2-photon in-vivo
• Model
Femtonics FEMTO3D-RC
• Microscope Equipped With
1. 1 LED source (with 4 LEDs), 4 filters sets (DAPI, GFP, mCherry and Cy5 filters), 1 sCMOS camera (Hamamatsu Orca Flash 2.8)
2.3 femtosecond lasers: Coherent Chameleon Vision 2, Coherent Chameleon Ultra 2 & NEW Spark Alcor 1064 nm usable simultaneously with 2 scanners (1 resonant & 1 galvanometric) for simultaneous imaging and photomanipulation.
3. 2 positions objective slider (available objectives: Nikon 5X dry, Olympus 20X 1.0 W & Nikon 25X 1. 1 W)
4. 2 upper GaAsP NDD detectors + 2 lower GaAsP NDD detectors, with GFP and mCherry filter sets. 1 trans PMT with Dodt contrast
5. Motorized stage allowing to use tissue slices or entire animals (mouse or rat). Anesthesia system available for in vivo imaging.
6. Z-stack imaging: Objective piezo system for a fast and precise scan in z with the possibility to scan 3D regions of interest
7. Different devices triggered by the microscope:
CD BioSciences' In-Vivo 2-Photon Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.