Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon N-SIM Super-Resolution Microscope-based confocal microscopy imaging services.
• Objectives
1. Nikon Apo TIRF 100× NA 1.49 oil immersion objective, working distance 0.12 mm. A correction collar allows the objective to be accommodated to coverslips with thickness between 0.13 and 0.20 mm.
2. Nikon CFI Plan Apo IR 60× WI (NA 1.27, WD 0.16 - 0.18 mm) water immersion
• Camera
1. Andor iXon3 DU-897E single photon detection EMCCD camera. This camera offers a resolution of 512 × 512 pixels with a gigantic on-chip pixel size of 16 × 16 µm and a pixel well capacity of 160,000 electrons. The chip is cooled down to -100℃ and 35 full frames can be read out per second.
The Nikon N-SIM structured illumination microscope on a Nikon Ti inverted microscope is equipped with 4 lasers (405, 488, 561, 640 nm) , covering a wide range of fluorescent dyes.
CD BioSciences' Nikon N-SIM Super-Resolution Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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