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Imaging and Analysis Services—Leica TCS-SP5 AOBS Confocal Microscope

CD BioSciences offers a unique range of high-end custom fluorescence microscopes, small animal imaging devices, and fluorescent probes to perform fluorescence-based measurements in a variety of spatial (nm to cm), and temporal (ns). Our laboratories offer consultancy services and support the application of novel spectroscopic methods and advanced microscopy techniques to achieve high spatial and temporal resolution nanotechnology from whole in vivo animal imaging to sub 70 nm imaging using super-resolution. In addition to using state-of-the-art specialist instrumentation, we train researchers in the use of advanced imaging techniques that are used to study macromolecules, and cell dynamics and characterize biological materials with nanometric precision.

Name of the Instrument/Platform

Leica TCS-SP5 AOBS Confocal Microscope

MANUFACTURER:
Leica

Introduction

With its proven, high-efficiency SP detection (five channels simultaneously) and AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed.

Feature Highlights

1. Leica Inverted DMI6000 microscope stand.
2. DIC, 6 PMTs.
3. CO2/temperature controlled enclosure for time-lapse imaging of live cells.
4. Single photon lasers (attenuated using acousto-optical tuning filters (AOTFs)).
5. Argon laser:excitation lines at 458, 476, 488, 496 and 514 nm (blue-cyan).
6. 3 helium-neon lasers: excitation lines at 543 (green), 594 (orange) and 633 (far red) nm.
7. 405 nm violet (near UV) laser for near UV excitation.
8. Acousto-Optical Beam Splitter (AOBS).

Applications

1. Fluorescence imaging at all length scales: from single-molecule detection to in vivo small animal imaging.
The field of optical microscopy imaging is experiencing major technical advances which are allowing cell biologists and physicians to visualize a new, dynamic, sub-cellular world where genes and gene products interact in space and time, in health and disease with nanometer-accuracy.
2. Micro-and nano-scale imaging.
The facility currently provides: wide-field fluorescence imaging microscopy, stereo microscopy, iterative deconvolution and computationally derived optical sectioning, confocal one-photon and two-photon laser scanning confocal microscopy imaging, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), time-correlated-single-photon-counting (TCSPC), near-infrared (NIR) imaging, and a super-resolution Stimulated Emission Depletion (STED) confocal microscope for probing biological and artif

Summary

Main Use: Standard and fast scanning confocal microscopy through the visible spectrum
The Leica TCS SP5 Confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. The system provides the full range of scan speeds at the highest resolution.

CD BioSciences offers Leica TCS-SP5 AOBS Confocal Microscope-based imaging and analysis services to researchers from all over the world. Our solutions will fully meet your experimental needs. We sincerely look forward to establishing a partnership with you. If you have any questions, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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