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Imaging and Analysis Services—Leica Confocal and Multiphoton TCS SP2 MP-FLIM

CD BioSciences offers a unique range of high-end custom fluorescence microscopes, small animal imaging devices, and fluorescent probes to perform fluorescence-based measurements in a variety of spatial (nm to cm), and temporal (ns). Our laboratories offer consultancy services and support the application of novel spectroscopic methods and advanced microscopy techniques to achieve high spatial and temporal resolution nanotechnology from whole in vivo animal imaging to sub 70 nm imaging using super-resolution. In addition to using state-of-the-art specialist instrumentation, we train researchers in the use of advanced imaging techniques that are used to study macromolecules, and cell dynamics and characterize biological materials with nanometric precision.

Name of the Instrument/Platform

Leica Confocal and Multiphoton TCS SP2 MP-FLIM

MANUFACTURER:
Leica

Introduction

The FLIM method: The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon. The emission of a fluorescence photon from a fluorophore does not occur at a fixed time. Instead, a distribution of times is observed, which can be described by an exponential decay function. The characteristic time constant of this decay, the fluorescence lifetime, is in the range of a few picoseconds (10-12 s) to several tens of nanoseconds (10-9 s).

Feature Highlights

1. Leica DMIRE2 inverted microscope stand.
2. Combined multi-photon/fluorescence lifetime imaging.
3. Ultrafast pulsed Spectra-Physics Mai-Tai Ti:S laser (tunable from 710 to 990 nm for IR excitation).
4. 2 non-descanned detectors (NDDs).
5. Single photon lasers (attenuated using acousto-optical tuning filters (AOTFs)).
6. Argon laser: excitation lines at 458, 476, 488, 496 and 514 nm(blue-cyan).
7. 3 helium-neon lasers: excitation lines at 543 (green), 594 (orange) and 633 (far red) nm.
8. Software modules for fluorescence (or Förster) resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP).
9. Acousto-Optical Beam Splitter (AOBS).
10. Computer attached with Becker-Hickl software for time correlated single photon counting (TCSPC) for FLIM data collection and analysis.

Applications

1. Fluorescence imaging at all length scales: from single-molecule detection to in vivo small animal imaging.
The field of optical microscopy imaging is experiencing major technical advances which are allowing cell biologists and physicians to visualize a new, dynamic, sub-cellular world where genes and gene products interact in space and time, in health and disease with nanometer-accuracy.
2. Micro-and nano-scale imaging.
The facility currently provides: wide-field fluorescence imaging microscopy, stereo microscopy, iterative deconvolution and computationally derived optical sectioning, confocal one-photon and two-photon laser scanning confocal microscopy imaging, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), time-correlated-single-photon-counting (TCSPC), near-infrared (NIR) imaging, and a super-resolution Stimulated Emission Depletion (STED) confocal microscope for probing biological and artif

Summary

Main Use: Standard confocal microscopy with slides and non-standard specimens, multiphoton microscopy and fluorescence lifetime imaging
The Leica DMIRE2 is the ideal combination of motorization, automation, high stability, optimized system compatibility, ergonomics and user convenience. And yet again, we have proved that Leica is not only keeping pace with progress, but is actually setting the pace.

CD BioSciences offers Leica Confocal and Multiphoton TCS SP2 MP-FLIM-based imaging and analysis services to researchers from all over the world. Our solutions will fully meet your experimental needs. We sincerely look forward to establishing a partnership with you. If you have any questions, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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