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Imaging and Analysis Services—Cambridge Research and Instrumentation (CRi) Maestro 2 In Vivo Small Animal Imaging System

CD BioSciences offers a unique range of high-end custom fluorescence microscopes, small animal imaging devices, and fluorescent probes to perform fluorescence-based measurements in a variety of spatial (nm to cm), and temporal (ns). Our laboratories offer consultancy services and support the application of novel spectroscopic methods and advanced microscopy techniques to achieve high spatial and temporal resolution nanotechnology from whole in vivo animal imaging to sub 70 nm imaging using super-resolution. In addition to using state-of-the-art specialist instrumentation, we train researchers in the use of advanced imaging techniques that are used to study macromolecules, and cell dynamics and characterize biological materials with nanometric precision.

Name of the Instrument/Platform

Cambridge Research and Instrumentation (CRi) Maestro 2 In Vivo Small Animal Imaging System

Introduction

The Maestro 2 system is fast: acquire full spectral images in only seconds. It is also flexible: image any fluorophore which emits beyond 500 nm. Image multiple fluorophores, even if they overlap spectrally and spatially. And the Maestro 2 is sensitive: up to 300 times more sensitive than monochrome!

Feature Highlights

1. Fluorescence observation and spectral unmixing of whole mice.
2. Same LCTF technology as the NUANCE system, but applied to whole living mice in the Maestro.
3. Xenon arc lamp for excitation and 7 excitation/emission sets for 500 nm to 950 nm imaging.
4. Dynamic Contrast Enhancement application for image rendering.
5. PC with CRi Maestro software similar to CRi NUANCE software.

Applications

1. Fluorescence imaging at all length scales: from single-molecule detection to in vivo small animal imaging.
The field of optical microscopy imaging is experiencing major technical advances which are allowing cell biologists and physicians to visualize a new, dynamic, sub-cellular world where genes and gene products interact in space and time, in health and disease with nanometer-accuracy.
2. Micro-and nano-scale imaging.
The facility currently provides: wide-field fluorescence imaging microscopy, stereo microscopy, iterative deconvolution and computationally derived optical sectioning, confocal one-photon and two-photon laser scanning confocal microscopy imaging, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), time-correlated-single-photon-counting (TCSPC), near-infrared (NIR) imaging, and a super-resolution Stimulated Emission Depletion (STED) confocal microscope for probing biological and artif

Summary

Main Use: Spectral unmixing imaging of fluorescence signals in small animals or other macroscale specimens
The Maestro 2 system combines high sensitivity with advanced animal-handling features and user-friendly time-saving operation. Focus, adjust the specimen stage, or change filters at the click of a button. Broadband mode allows increased sensitivity, while narrowband mode allows higher accuracy for improved spectral separation.

CD BioSciences offers Cambridge Research and Instrumentation (CRi) Maestro 2 In Vivo Small Animal Imaging System-based imaging and analysis services to researchers from all over the world. Our solutions will fully meet your experimental needs. We sincerely look forward to establishing a partnership with you. If you have any questions, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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