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Imaging and Analysis Services—ALMS Confocal MicroALEX Fluorescence Correlation Spectroscopy

CD BioSciences offers a unique range of high-end custom fluorescence microscopes, small animal imaging devices, and fluorescent probes to perform fluorescence-based measurements in a variety of spatial (nm to cm), and temporal (ns). Our laboratories offer consultancy services and support the application of novel spectroscopic methods and advanced microscopy techniques to achieve high spatial and temporal resolution nanotechnology from whole in vivo animal imaging to sub 70 nm imaging using super-resolution. In addition to using state-of-the-art specialist instrumentation, we train researchers in the use of advanced imaging techniques that are used to study macromolecules, and cell dynamics and characterize biological materials with nanometric precision.

Name of the Instrument/Platform

ALMS Confocal MicroALEX Fluorescence Correlation Spectroscopy

Feature Highlights

1. Confocal or wide-field geometry.
2. Olympus IX71 microscope stand.
3. 3 alternating lasers (blue, green and red) for FRET-based measurement in solution.
4. Alternating Laser Excitation spectroscopy: ALEX.
5. 3 APD detectors.
6. Fast time-correlated counting board (6 ns-resolution).
7. EMCCD camera and PC computer for recording and processing data.
8. Data can be plotted with stoichiometry vs. FRET to show different fluorochrome FRET combinations much like a plot from a flow cytometer.

Applications

1. Fluorescence imaging at all length scales: from single-molecule detection to in vivo small animal imaging.
The field of optical microscopy imaging is experiencing major technical advances which are allowing cell biologists and physicians to visualize a new, dynamic, sub-cellular world where genes and gene products interact in space and time, in health and disease with nanometer-accuracy.
2. Micro-and nano-scale imaging.
The facility currently provides: wide-field fluorescence imaging microscopy, stereo microscopy, iterative deconvolution and computationally derived optical sectioning, confocal one-photon and two-photon laser scanning confocal microscopy imaging, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), time-correlated-single-photon-counting (TCSPC), near-infrared (NIR) imaging, and a super-resolution Stimulated Emission Depletion (STED) confocal microscope for probing biological and artif

Summary

Main uses: Alternating laser excitation spectroscopy. FRET ratio and stoichiometry measurements at very low light levels
The MicroALEX-FCS is a custom built single-molecule microscopy set-up for alternating laser excitation spectroscopy

CD BioSciences offers ALMS Confocal MicroALEX Fluorescence Correlation Spectroscopy-based imaging and analysis services to researchers from all over the world. Our solutions will fully meet your experimental needs. We sincerely look forward to establishing a partnership with you. If you have any questions, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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