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Fermentation Service—Microfluidizer Disrupts

CD BioSciences fermentation services are tailored to the needs of our customers. We have the high density fermentation technology and process optimization to provide you with transformed bacterial cytoplasm (bacteriophage), as well as gram-level homologous recombinant proteins with up to 98% purity. Customers can choose the right fermentation solution for their needs.

Name of the Instrument/Platform

Microfluidizer Disrupts

Introduction

This device can conveniently process relatively large volumes of cell suspension (typically up to approximately 1 liter) because it operates in continuous flow mode, unlike the French Pressure Cell, which must be re-filled for every 35 mL of suspension.
Since the Microfluidizer does not always shear the DNA released when cells break, it is recommended to add DNAase (and Mg2+) prior to processing to avoid high viscosity conditions.

Summary

The Microfluidizer disrupts ("breaks") the cells of microorganisms by elevating the cell suspension to a high pressure and then allowing it to escape back to ambient pressure through a small orifice.

CD BioSciences' Microfluidizer Disrupts-based fermentation services can be applied to protein purification. If you need technical support in this area, please feel free to contact us.

For research use only, not intended for any clinical use.
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