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Electron microscopes can excite electrons to form images that magnify micron and nanostructures up to 10 million times, providing amazing levels of fine magnification and allowing researchers to observe even individual atoms. CD BioSciences can provide innovative solutions for electron microscopy and microanalysis. We offer FEI Tecnai G2 20 TWIN instruments that allow customers to combine high-resolution imaging with physical, elemental, chemical and electrical analysis to obtain usable data from the widest range of samples.
The Tecnai is also equipped with the iCorr which is a fully integrated wide-field fluorescence microscope. iCorr allows to image sequentially with fluorescence and electron microscopy, without manually changing the sample between the 2 modes (Correlative Light and Electron Microscopy: an Impression and the Solutions).
Specifications
1. Filament type: LaB6 emitter
2. Accelerating Voltage (HT): 40KV - 200kV
3. Magnification range: 25× - 700.000×
1. Correlative microscopy
is done in combination with the iCorr. By tilting the sample holder 90° degrees, you can fast localize precise areas of interest using the fluorescence microscope and then tilt the holder back to 0° degrees and look for ultra-structural details with the electron microscope.
The fluorescence signal in an image identifies the positions where labeled molecules or structures of interest are located. These positions can be stored with a click of a mouse using the iCorr software. Using a shared coordinate system, these positions of interest are automatically located and imaged in TEM mode to gather ultra-structural detail with a single mouse click. These TEM images are automatically superimposed on the LM grid overview with precise accuracy, delivering fast, high quality correlated results.
2. Cryo-applications
are used to examine frozen suspensions of single particles, 2D protein crystal sheets and frozen-hydrated sections.
The microscope is equipped with retracta
Tomography
is used to obtaining detailed 3D structures of sub cellular macromolecular objects. The electron beam is passing through the sample at incremental degrees of rotation (max ± 65 degrees) around the center of the target sample (tilt axis). The acquired tilt series is used to assemble a three dimensional image of the target and this is done with the Xplore3D software package.
This application consists of three modules; one for the acquisition of tomography data on the Tecnai (tilt series acquisition), one for alignment and reconstruction of the acquired tilt series (Inspect3D), the last one is to visualize the reconstructed 3D image (Amira), rapid exploration and analysis of 3D data and generating polygonal and tetrahedral 3D models for advanced visualization and simulation.
Another option for reconstruction of your acquired tilt series are the following software packages: IMOD and FiJi (ImageJ in combination with java3D and special plug-ins). The image post processing softwar
The FEI Tecnai G2 20 TWIN is a 200 kV Transmission Electron Microscope.
It has an advanced operating system: all microscope components - the electron gun, the optical elements, the vacuum system and the stage - are digitally controlled by the Tecnai user interface on the microscope PC. Data acquisition is done with the bottom mounted FEI High-Sensitive (HS) 4k × 4k Eagle camera or the Side-mounted Olympus 1.3k × 1K Megaview G2 camera. Both camera's are embedded in the Tecnai user interface and can be automated for acquisition processes, like Cryo with low-dose technique & tomography.
CD BioSciences provides FEI Tecnai G2 20 TWIN-based electron microscopy imaging services to our clients. If you are interested, please feel free to contact us.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.