CryoEM Imaging Service—Tecnai F20 Electron Microscope

CryoEM is a microscopy technique that applies a freeze-fixation technique to obtain the structure of biological macromolecules by using transmission electron microscopy to view samples at low temperatures. The sample is cryo-fixed and placed in an electron microscope where electrons are scattered in the sample and nearby ice. CD BioSciences uses a detector and lens system to image and record the scattered signals, which are then processed using 3D reconstruction techniques to obtain the 3D structure of the sample.

Name of the Instrument/Platform

Tecnai F20 Electron Microscope

Feature Highlights

1. 200 kV acceleration voltage
2. Field-emission gun (FEG) electron source
3. Gatan K2 direct electron detector; 3,712 × 3,840 pixels; 5 µm pixel size; 400 frames/sec; DQE up to 0.8 in electron counting mode.
4. SerialEM software for manual and semi-automated, low-dose acquisition for single-particle and tomographic data.
5. Gatan 626 high-tilt cryo-sample holder, tilt range up to ±70°

Summary

The Cryo-Electron Microscopy Facility provides capabilities for high-resolution electron microscopy and tomography of stained and unstained specimens. Cryo-EM allows the observation of biological samples in their native environment, in the absence of the distortions and artifacts associated with traditional sample preparation methods, and is suitable for proteins and protein complexes, viruses, fibers, liposomes and intact prokaryotic cells up to about 1µm thickness. The Gatan K2 direct electron detector and SerialEM automated acquisition software enables near-atomic resolution imaging for suitable specimens as small as 150 kDa.

CD BioSciences offers its customers the CryoEM imaging service based on Tecnai F20 Electron Microscope to help analyze the 3D structure and microstructure of samples. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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