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Confocal Microscope Imaging Service—M42-LSM980 Axio Observer (Inverted)

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with M42-LSM980 Axio Observer (Inverted)-based confocal microscopy imaging services.

Name of the Instrument/Platform

M42-LSM980 Axio Observer (Inverted)

Introduction

1. Widefield Illumination
1) LED
2) LED for brightfield
2. Lasers for Confocal Imaging
1) 405 nm
2) 445 nm
3) 488 nm
4) 514 nm
5) 561 nm
6) 639 nm
3. Filters
1) CH1: 380-736
2) CH2: 380-736
3) CHS1-4 GaAsP: 409-694
4) CHD: T-PMT (transmitted light)
4. Objectives
1) 10×/0.3 plan-apochromat DIC
2) 20×/0.8 plan-apochromat DIC
3) 25×/0.8 LD LCI plan-apochromat DIC
4) 40×/1.1 LD C-Apochromat Water
5) 40×/1.3 EC plan-apochromat Oil DIC
6) 63×/1.4 plan-apochromat Oil DIC
5. Additional Devices
1) Incubation with CO2
2) XY Motorized Stage
3) Piezo Z stage insert
4) Multiple Position Acquisition
5) High Dynamic Range acquisition (HDR)
6) FRAP
7) Tiles

Summary

The M42 system is an inverted point laser scanning confocal microscope - LSM980. It is a spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM980 features 4 highly sensitive GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2× SNR as compared to standards PMTs.
The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.

CD BioSciences' M42-LSM980 Axio Observer (Inverted)-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.
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