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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Carl Zeiss LSM 880 Airyscan-based confocal microscopy imaging services.
The instrument is suitable for three-color co-localization with minimized spectral overlap, overlay of fluorescence onto bright-field images, tiling of images, 3D reconstruction, Fluorescence Recovery After Photo-bleaching (FRAP), and Fluorescence Resonance Energy Transfer (FRET). Further, the instrument supports microfluorometic measurements including programmed time-lapse imaging, ratio-metric imaging of pH and Ca2+ sensitive dyes, and line scans for fast events such as Ca2+ sparks.
The Carl Zeiss LSM 880 is outfitted with a temperature-controlled 8-line superfusion setup for live-tissue microfluorometry including pH and Ca2+ measurements in conjunction with fast solution changes at 37℃.
The confocal microscope, Carl Zeiss LSM 880 Airyscan, consists of an inverted microscope outfitted with six objectives, 2.5×, 5×, 10×, 20×, 40× (1.3 NA Oil) and 63× (1.4 NA Oil), six lasers, 405 (blue), 458 (blue), 488 (cyan), 514 (green), 561 (green) and 633 nm (red), three fluorescence emission detectors and one transmission detector. One fluorescence detector is a gallium arsenide phosphide (GaAsP) detector, which is significantly more sensitive than conventional photo-multiplier tubes (PMTs), making it ideal for low SNR samples. Airyscan mode allows for superresolution imaging, which provides greater resolution in x, y and z.
CD BioSciences' Carl Zeiss LSM 880 Airyscan-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.