Confocal Microscope Imaging Service—Zeiss Cell Observer Dpinning Disc Confocal with FRAP and TIRF

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Zeiss Cell Observer Dpinning Disc Confocal with FRAP and TIRF-based confocal microscopy imaging services.

Name of the Instrument/Platform

Zeiss Cell Observer Dpinning Disc Confocal with FRAP and TIRF

MANUFACTURER:

Zeiss

Introduction

1. Total internal reflection fluorescence (TIRF) microscopy with 405 nm, 488 nm and 561 nm excitation
2. Fluorescence recovery after photobleaching (FRAP), DirectFRAP-module
3. Fully motorized inverted microscope and stage for multi-position imaging
4. Suitable sample formats: slides, slide chambers, 35 mm and 50 mm dishes, 6-well and 24-well plates

Required Training

1. Excitation: 405 nm, 488 nm and 561 nm lasers, metal-halide lamp
2. Detection: Hamamatsu ImagEM EM-CCD and ORCA-R2 CCD cameras, fluorescence filters:
3. Spinning disc: DAPI (BP 450/50 nm), GFP (BP 525/50 nm) and mCherry (BP 629/62 nm)
4. TIRF: Filter Set 74 HE (DBP 525/31 nm + 616/57 nm) and Filter Set 76 HE (TBP 459 nm + 525 nm + 608 nm)
5. EPI: DAPI (BP ex.359/24 nm, em.445/50 nm), GFP (BP ex.470/20 nm, em.525/50 nm), Texas Red (ex.560/20 nm, em. 630/75 nm)
6. Transmitted light detection (DIC 25× -100× objectives/Phase contrast 10× objective)
7. Spinning disc unit: Yokogawa CSU-X1
8. Objectives:
1) Plan NeoFluar 10× /0.30 Ph1 (air)
2) LD LCI Plan-Apochromat 25× /0.8 Imm. Korr DIC (water, oil, glycerine)
3) EC Plan NeoFluar 40× /0.75 DIC (air)
4) alpha Plan-Apochromat 63×/1.46 Korr DIC (oil)
5) alpha Plan-Apochromat 100× /1.46 DIC (oil)
6) objectives from Zeiss LSM 780 on request
9. Autofocus: software based or hardware-based DefiniteFocus
10. Incubator: Top stage incubator for live

Applications

For live-cell imaging, molecular dynamics (FRAP) and cell-surface events detection (TIRF).

CD BioSciences' Zeiss Cell Observer Dpinning Disc Confocal with FRAP and TIRF-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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