Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Zeiss Elyra PS.1 Super Resolution Microscope.
Structured Illumination Microscopy (SIM) relies on light interfences creating moire fringes and improves the resolution by a factor of 2 (xy: 100 nm; z: 250 nm). Here are some recommendations for sample preparation for SIM.
Localization microscopy techniques such as dSTORM and PALM take advantage of the blinking properties of some fluorophores to calculate their precise location in a sample. The resolution is greatly improved (xy: 40nm ; z: 100nm via TIRF).
• Specifications
1. Imaging:
1) Widefield / SIM / PALM / dSTORM / TIRF
2) SIM: sCMOS PCO.edge
2. Cameras:
1) PALM/dSTORM: cooled EMCCD Andor - iXon DU897
2) INVERTED
3. Stand :Zeiss AxioObserver
4. Stage: Motorized in XYZ
5. Software: Zeiss Zen Black 2012
• Objectives
1. Objective: EC Plan Neofluar, Plan-Apochromat, C-Apochromat, Plan-Apochromat, alpha Plan-Apochromat, alpha Plan-Apochromat
2. Mag. / NA: 10× / 0.3, 20× / 0.8, 63× / 1.2, 63× / 1.4, 100× /1.46, 100× / 1.57
3. Medium: Air, Air, Water, Oil, Oil, Oil-HI
4. Contrast: DIC
Elyra is a super resolution microscope with Structured Illumination Microscopy (SIM), Total Internal Reflection Fluorescence (TIRF) and Single Molecule Localization Microscopy (PALM or dSTORM for example).
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.