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Optical Microscope Imaging Service—Zeiss Cell Observer SD

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Zeiss Cell Observer SD.

Name of the Instrument/Platform

Zeiss Cell Observer SD

MANUFACTURER:
Zeiss

Introduction

Microscope
Fully motorized inverted research microscope Zeiss Cell Observer. Z1 equipped with
1. high precision motorized z-drive (min. step length 10 nm)
2. external microscope control unit with TFT display and separate z-drive knob for noncontact focusing
3. motorized revolving nosepiece with Zeiss Definite Focus system (realized via 835 nm LED)
4. motorized xyz-stage PZ-2000 XYZ from Applied Scientific Instrumentation:
1) travel range: 120 × 110 mm
2) resolution: 88 nm
3) repeatability: < 0.7 µm
4) with high-precision piezo z-axis top plate
a. travel range: 150 µm
b. resolution: 2.2 nm
c. repeatability: +/- 5 nm
5. 20 W Vis-LED (400 - 700 nm) as transmission light
6. Uniblitz shutter for transmission light with controller VCM-D1
1) Long distance condenser (working distance 26mm, NA 0.55) with motorized 6 position turret equipped with
2) DIC prism DICII/0.55 plus polarizer
3) DIC prism DICIII/0.55 plus polarizer
7. motorized filter cassette
8. motorized Optovar revolver with:
1) Op

Summary

This setup is a live cell imaging platform for dual-color high-speed confocal imaging using the CSU-X1 spinning disc technology from Yokogawa and two highly-sensitive electron multiplying cameras. Almost the entire microscope from filter wheels to sample incubation parameters are computer-controlled via the Zeiss Zen 2012 software. The Definite Focus system from Zeiss allows for long-term time-lapse experiments without any focus drifts. The DirectFRAP unit from Zeiss is able to switch fluorophores exactly at the same time by continuous diaphragm imaging.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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