Optical Microscope Imaging Service—Oxford NanoImaging-Nanoimager (Super-resolution)

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Oxford NanoImaging-Nanoimager (Super-resolution).

Name of the Instrument/Platform

Oxford NanoImaging-Nanoimager (Super-resolution)

Introduction

The instrument supports various modes of operation: single-molecule localization-based super-resolution for quantitative cellular imaging, TIRF and epifluorescence for diffraction-limited fluorescence imaging, single-molecule FRET for measuring molecular interactions in the 2-10 nm range and single particle tracking PALM in cells.

Feature Highlights

Achievable resolution
• Lateral: exceeding 20 nm
• Axial: exceeding 50 nm
Simultaneous imaging channels
• 2 (< 10 nm channel mapping accuracy)
Total number of imaging colors
• Up to 4 lasers
Field of view
• 50 μm × 80 μm per channel
Software features
• Real-time 3D localization analysis and rendering (sCMOS optimized)
• Real-time FRET trace analysis
• Clustering and co-localization analysis
• Residual drift correction
• Scripting interface and OMERO compatibility
Acquisition speed
• 100 fps full frame
• 5 kHz with frame height cropped to 2%
3D imaging technique
• Astigmatism
Time for super-resolution full frame
• Seconds to minutes (number of localizations and laser power dependent)

Applications

Tracking single molecules and vesicles in cells
1. Quantitative cellular imaging
2. Molecular mechanisms and interactions
3. Protein complex assembly
4. Epigenetic mapping
5. DNA-PAINT shows sub-20nm resolution
6. Single-molecule FRET
7. Exosomes and microvesicles
8. Neuronal imaging

Summary

The Nanoimager achieves super-resolution by single-molecule localization (dSTORM and PALM).
These techniques involve localizing only subsets of fluorophores in consecutive frames with high precision (typically better than 20 nm laterally and 50 nm axially), and reconstructing an image from the positions of the localizations.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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