Optical Microscope Imaging Service—Bruker Investigator Upright Multiphoton Microscope

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Bruker Investigator Upright Multiphoton Microscope.

Name of the Instrument/Platform

Bruker Investigator Upright Multiphoton Microscope

MANUFACTURER:

Bruker

Introduction

1. Modalities:
1) Multiphoton microscopy
2) Fluorescence Lifetime Imaging Microscopy (FLIM)
2. Laser:
Coherent Chameleon Discovery (Synchronized 680-1300nm Variable, and 1040nm Fixed Lines)
Resonance Energy Transfer (FRET)
3. Directors:
Two Hamamatsu H10770 GaAsP tubes
4. Objectives Available:
16× / 0.8 NA (water dipping) (wd=3 mm)
20× / 0.75 NA (wd=1 mm)
40× / 0.8 NA (water dipping) (wd=2 mm)
60× / 0.95 NA (wd=0.20-0.11 mm)
5. Fluorescence Lifetime:
Becker-Hickl SPC-150 TCSPC
6. Microscope:
Custom Bruker upright
7. Stage:
Microscope moves in XY and Z. Motorized sample stage is available
8. Piezo Z:
250 micron objective mount piezo is available for high speed imaging.
9. Multiphoton Emission Filter-sets:
Green/Red: ET525/70m-2p and ET595/50m-2p and T565lpxr dichroic mirror
SHG/Green: ET460/40m-2P and ET525/50m-2P with T495LPXR Dichroic mirror
10. Epi Fluorescence Filter set (not for imaging):
ET-GFP (FITC/CY2), ET-DSRed (TRITC/CY3)

Summary

The Bruker Ultima Investigator is an upright multiphoton microscope designed for both intravital and in vitro microscopy. The microscope body itself moves is motorized in X, Y, and Z so samples may remain fixed in place. The objective nose piece tilts up to 90 degrees for off-axis imaging. The laser is a Coherent Chameleon Discovery with two synchronized laser lines, a 680-1300 nm tunable line and a 1040 nm fixed. This system has two GaAsP detectors, standard galvos and resonant scanners, and a Becker-Hickl SPC-150 module for lifetime imaging (FLIM). A GPIO box allows for input and output triggers and analog signals generated by the system enable interaction with external devices for sensory stimulation, electrical recording, and other experimental devices.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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