Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Olympus TIRF 1-Line with JPK AFM.
Microscope
Inverted research microscope Olympus IX-71 equipped with
1. manual filter cassette
2. manual revolving nosepiece
3. manual z-drive
4. manual xy-stage
5. 100 W halogen lamp as transmission light source
6. Mid-long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55)
7. Optional optovar lens: 1.6×
Fluorescence Illumination
1. 488 nm laser diode, max. power 60 mW (LuxX 488-60, Omicron)
2. 561 nm diode-pumped solid state laser, max. power 150 mW (Cobolt Jive 561, Cobolt)
3.642 nm laser diode, max. power 140 mW (LuxX 642-140, Omicron)
4. All laser lines are coupled into a single mode fiber which is connected to a home-built single-line TIRF condenser
Electron Multiplying CCD Camera
Andor iXon3 897
1. Electron multiplying back-illuminated frame transfer CCD
2. forced-air cooled at -85℃
3. quantum efficiency: > 90% between 500 - 700 nm.
4. Effective number of pixel: 512 × 512
5. Pixel size: 16 µm × 16 µm
6. Pixel depth: True 14-bit
7. Pixel clock-rate:
1) EM-
This setup is a combination of a JPK Nanowizard atomic force microscope with a home-build TIRF condenser on a Olympus IX-71 microscope. It is equipped with three powerfull laser-lines (488nm, 561 nm, 642 nm) and a highly-sensitive EMCCD camera to combine AFM with single molecule imaging.
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.