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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on LeicaSP8/STED.
Our STED is built on an inverted fully motorized confocal microscope from Leica (SP8) with a hardware based focus drift-correction. The SP8 is configured with four detectors (two PMT's and two high sensitive HyD's) and two scanners (conventional and 12 kHz resonant). Six laser lines (405 nm, 458 nm, 488 nm, 514 nm, 555 nm, and 640 nm) and a white light laser (WLL) tunable from 470 nm to 670 nm are available for excitation. Suppression of laser light and improvement of the resolution in the STED mode is achieved by time gating available for the two HyD's. The SP8/STED 3X achieves resolutions below the diffraction limit in lateral as well as axial directions. STED depletion is done with a 592 nm or a 775 nm laser. For excitation in the STED mode the 405 nm, 458 nm, 488 nm, 515 nm or the WLL can be chosen. Under ideal conditions a resolution of 30 nm to 50 nm (lateral) and 120 nm (axial) can be achieved. This STED setup is ideally suited for two or three color STED with conventional "gree
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.