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Optical Microscope Imaging Service—Laser Microdissection

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Laser Microdissection.

Name of the Instrument/Platform

Laser Microdissection

Introduction

This widefield microscope is equipped with:
1. 3 sets of filters for imaging (Blue/Green/Red)
2. a laser microdissection unit - dissection and recovery of cells or subcellular compartments,
3. optical tweezers - mechanical manipulation of cells or samples without contact by using an infra-red laser,
4. an incubator for live cell imaging (time lapse).
• Specifications
5. Imaging: Widefield
6. Specificity:
1) Laser Microdissection
2) Optical Tweezers
7. Cameras:
1) AxioCamIcc1 version3
2) AxioCam MRm version3
8. Stand :
1) INVERTED
2) Zeiss Axio Observer Z1
9. Stage : Motorized in XYZ
10. Software: Robo software 4.2 Pro
11. Incubator: Incubator (temperature + gas control) XL PALM S1 system

Protocol

1. RNA handling
2. RNA extraction from frozen sections
3. RNA extraction from FFPE sections
4. RNA Quality
5. DNA handling
6. Chromosome preparation
7. Protein handling
8. Cell recultivation
9. Immunofluorescence on frozen sections
10. FISH hybridization

Summary

Microdissection allows one to select and isolate part of a sample and use it for further studies (RT-PCR, protein analysis, selection of live cells). Laser microdissection can also be used with live samples to study recovery after a cut, protein recruitment after photodamage… The system is paired with optical tweezer to manipulate samples.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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