Molecular Characterization Service—Isothermal Titration Calorimeter

The characterization of molecules is an important part of the process, enabling the physical and chemical properties of molecules to be resolved. CD BioSciences has excellent analytical staff, years of analytical experience, and cutting-edge instrumentation. Using LC-MS, multiple HPLC, and other techniques, we can accurately quantify/characterize the DAR of coupled drugs, residual small molecules, endotoxins, and more.

Name of the Instrument/Platform

Isothermal Titration Calorimeter

Introduction

By measuring the amount of heat released or taken up during each reaction in a titration experiment, the equilibrium binding affinity and the stoichiometry are derived; in addition, ITC is the only technique able to directly measure the thermodynamic parameters of binding (enthalpy, entropy and heat capacity changes)-providing therefore invaluable insights into the thermodynamic forces governing biomolecular interactions.

Feature Highlights

1. Affinity constants from 100 to ~109 M-1 can be measured by ITC.
2. The sample cell volume is approximately 200 μL; minimum sample volume is 290 μL.
3. The syringe volume is 40 μL.
4. The operating temperature range is 2℃ to 80℃.

Applications

The technique is suitable for analyzing protein-protein, protein-DNA/RNA interactions as well as interactions between biopolymers or synthetic macromolecules and small ligands.

Summary

The MicroCal (GE Healthcare) ITC200 isothermal titration calorimeter is a powerful tool for label-free analysis of macromolecular interactions in solution.

CD BioSciences offers its customers Isothermal Titration Calorimeter-based molecular characterization services that contribute to the process and drug development process of biomolecules. If you need our technical support, please contact us directly.

For research use only, not intended for any clinical use.

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