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The characterization of molecules is an important part of the process, enabling the physical and chemical properties of molecules to be resolved. CD BioSciences has excellent analytical staff, years of analytical experience, and cutting-edge instrumentation. Using LC-MS, multiple HPLC, and other techniques, we can accurately quantify/characterize the DAR of coupled drugs, residual small molecules, endotoxins, and more.
BLItz™ uses ForteBio's Dip and Read™ label-free assays. These direct binding assays take place on a disposable biosensor made from a biocompatible matrix that is uniform, non-denaturing and minimizes non-specific binding. Only molecules that bind directly to the biosensor surface are detected, providing exceptional specificity for individual applications, even in crude media.
The BLI (bio-layer interferometry) technology used by BLItz provides real-time data on protein interactions. BLItz emits white light down the biosensor, and then collects any light reflected back. Reflected wavelengths are affected by the thickness of the coating on the optical layer. Some wavelengths show constructive interference (blue), others show destructive interference (red).
This interference is captured by a spectrometer as a unique spectral signature and is reported in relative intensity units (nm). Any change in the number of molecules bound to the biosensor causes a shift in the
The BLItz is a micro volume instrument for characterizing the kinetics of macromolecular interactions using bio-layer interferometry with low cost disposable sensor probes.
CD BioSciences offers its customers BLItz Bio-layer Interferometer-based molecular characterization services that contribute to the process and drug development process of biomolecules. If you need our technical support, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.