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The Molecular & Cellular Analysis Core is designed to assist users with molecular and cellular analysis of tissues, and gene expression studies including, but not limited to, primer and mutant design, amplification and quantification of RNA or DNA products, cloning, and other molecular biology techniques. To achieve this, we are equipped with a comprehensive range of instruments. BioRad iCycler iQ rtPCR-based molecular and cellular analysis services can provide you with an unexpected experience.
1. Quantitative comparison of genes of interest in housekeeping genes in a single sample
2. Quantitative detection of gene expression in genetically modified organisms
3. Analysis of single nucleotide polymorphisms (SNPs)
4. When used in conjunction with laser capture microdissection, real time PCR provides an excellent technique for comparison of genetic expression in specific cell types such as cancer vs. normal cells from a single region of tissue.
Real time PCR, in conjunction with the appropriate fluorescent detection strategies, allows the initial amount of nucleic acid in a reaction to be determined. Advances in the technology have led to significant improvements in the accuracy of DNA quantitation and gene expression analysis. Quantitation is achieved by measuring the increase in fluorescence during the exponential phase of the PCR reaction. The system in the IRF is equipped for:
1. Three color analysis for amplification of three dependent targets
2. Taqman, Sybr Green, primer based system and molecular beacon analyses
3. Multiple sample block formats for 0.2 mL tubes, 96 well plates and 384 well PCR plates
4. Real time display of data acquisition
If you are interested in our BioRad iCycler iQ rtPCR-based molecular and cellular analysis services, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.