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Molecular and Cellular Analysis Services—BioRad BioPlex 100 and 200 Systems

The Molecular & Cellular Analysis Core is designed to assist users with molecular and cellular analysis of tissues, and gene expression studies including, but not limited to, primer and mutant design, amplification and quantification of RNA or DNA products, cloning, and other molecular biology techniques. To achieve this, we are equipped with a comprehensive range of instruments. BioRad BioPlex 100 and 200 Systems-based molecular and cellular analysis services can provide you with an unexpected experience.

Name of the Instrument/Platform

BioRad BioPlex 100 and 200 Systems

Required Training

The Bio-Plex suspension array is built around three core technologies:
1. The first is the family of fluorescently dyed microspheres, or beads, from Luminex Corp.
2. The second is a flow cytometer with two lasers and associated optics to measure biochemical reactions that occur on the surface of the microspheres.
3. The third is a high-speed digital signal processor to efficiently manage the fluorescent output.

Applications

1. Multiplex analysis of cytokines and phoshoproteins with prepared kits available from BioRad
2. Multiplex analysis of a variety of proteins by conjugating antibodies to beads via kits available from BioRad

Summary

The BioPlex system is a multiplex analysis system that permits the simultaneous analysis of up to 100 (or 200) different biomolecules (proteins, peptides, or nucleic acids) in a single microplate well. By multiplexing with the Bio-Plex system, researchers can dramatically increase the amount of useful information from rare or volume-limited samples such as mouse or rat serum, and decipher complex interrelationships among proteins involved in signal transduction pathways. The microplate platform allows the automated analysis of 96-well plates with a throughput of more than 1,800 assay points (for a 23-plex assay) in 30 minutes.

If you are interested in our BioRad BioPlex 100 and 200 Systems-based molecular and cellular analysis services, please contact us directly.

For research use only, not intended for any clinical use.
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