Microscope Imaging Service—Vectra Polaris

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Vectra Polaris-based confocal microscopy imaging services.

Name of the Instrument/Platform

Vectra Polaris

Introduction

Technical specifications
1. Wide Field Illumination
1) LED
2. Fluorescent lighting
1) LED
3. Filter sets:
1) DAPI, Opal 570, Opal 690
2) Opal 480, Opal 620, Opal 780
3) Opal 520
4. Translated to other dyes:
1) Opal 480 similar to Coumarin
2) Opal 520 similar to Alexa488, FITC, Cy2, GFP
3) Opal 570 similar to Alexa555, Cy3
4) Opal 620 similar to Alexa594, Texas Red, RFP
5) Opal 690 similar to Alexa660-700, Cy5
6) Opal 780 similar to Alexa750, Cy7
5. Multispectral range:
1) 440-780 nm
6. Camera:
1) Hamamatsu
7. Lenses:
1) 10×/NA = 0.2 = 1.0 µm/pixel
2) 20×/NA = 0.45 = 0.5 µm/pixel
3) 40×/NA = 0.75 = 0.25 µm/pixel
8. Software:
9. Vectra Polaris
10. Phenotype
11. Analysis: inFORM or HALO
12. File formats: qptiff. = MOTIF scan format, iM3. = Multispectral Field Format, tiff., png., jpeg.

Summary

With the slide scanner Vectra Polaris you can record wide field and fluorescence. Up to 80 slides can be scanned in one go. For fluorescence images, you can choose between two scan modes. If one wants to scan the whole slide, the slide is recorded with the fast MOTIF technology. Fields of interest are scanned using LCTF technology, a liquid crystal filter. In fluorescence, you can scan between 7 and 9 colors multispectrally, which can then be unmixed using software. Scanning of TMA slides is also possible.

CD BioSciences' Vectra Polaris-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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