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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with TCS SP5 Confocal Microscope-based confocal microscopy imaging services.
Technical details
1. Microscope stand: DM6000, upright configuration
2. Scanning head: SP5 II with Acousto-Optical Beam Splitter (AOBS) and resonant scanner (8 kHz)
3. Stage: motorized, inserts for slides, Petri dishes and microtiter plates
4. Detectors: 2 HyDs, 3 PMTs + TLD
5. Lasers:
6. 405 nm (cw 50 mW)
7. Argon laser (5 lines)
8. 561 nm
9. 633 nm
Objectives (default set):
1. HC PL APO CS 10×/0.40 Dry, WD: 2.2, DIC, 15506285)
2. HC PL APO CS 20×/0.70 ImmCorr, WD: 0.26-0.17, CCorr.DIC, (15506326)
3. HCX PL APO CS 40×/0.85 Dry, WD: 0.21, CCorr. (15506295)
4. HCX PL APO CS 63×/1.4 Oil, WD: 0.1, (15506188)
5. HCX PL APO CS 63×/1.30 Glyc, WD: 0.28, CCorr. (15506194)
6. HCX APO L 40×/0.80 Water, WD: 3.3, UV - V - I (15506155)
7. HCX APO L 63×/0.90 Water, WD: 2.2, UV - V - I (15506148)
• Visual observation of the fluorescent signal is possible by illuminating the samples with a metal halide lamp, however, the stand holds no camera for non-confocal image acquisition.
LAS Software Mod
The Leica TCS SP5 is a laser (point) scanning confocal microscope that enables imaging of transparent, medium thick samples via optical sectioning. Specimens/objects of interest should be fluorescently labelled for image acquisition.
Labelling protocols should be adapted to the existing laser lines that can excite the reporter dye molecules or fluorescent proteins.
CD BioSciences' TCS SP5 Confocal Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.