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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon TIRF/FRAP Microscope-based confocal microscopy imaging services.
Objectives:
1. Nikon Plan Fluor 10× NA 0.3 (working distance 16 mm) , corrected for 0.17 mm coverslips (= type #1.5).
2. Nikon Plan Fluor 20× NA 0.75 multi immersion (oil, glycerol, water; working distance 0.35 mm) , corrected for 0.17 mm coverslips (= type #1.5).
3. Nikon S Fluor 40× NA 1.30 oil immersion objective (working distance 0.22 mm) , corrected for 0.17 mm coverslips (= type #1.5).
4. Nikon Apo TIRF 60× NA 1.49 oil immersion objective (working distance 0.12 mm) adjustable for glas coverslips with a thickness between 0.13 and 0.21mm) , temperature adjustment for 23℃ and 37℃.
5. Nikon Plan Apo λ 100× NA 1.45 oil immersion objective (working distance 0.13 mm) , corrected for 0.17mm coverslips (= type #1.5).
Please note: not all the objectives are included in the system permanently. Some are only given to the users on request.
Cameras
1. Andor iXon Ultra DU-897U single photon detection EMCCD camera (for TIRF). This camera offers a resolution of 512 × 512 pixels with a gigan
The Nikon TIRF system with Nikon's H-TIRF module is an inverted and fully automated Ti2 microscope with objective TIRF illumination. A Nikon Perfect Focus System (PFS of the 4th generation) is included that continuously determines the distance to the coverslip and readjusts it if necessary (to counteract thermal drift or vibrations). For live cell imaging of mammalian cells, the system is equipped with an on-stage incubation chamber from TokaiHit, controlling temperature, CO2-concentration and humidity.
TIRF is short for Total Internal Reflection Fluorescence (Microscopy) : By directing a laser beam at an very shallow angel towards the coverslip on which cells adhere (and on the condition that the refractive index of the medium on that side is lower than that of glass and oil) , the light will not penetrate the coverslip but be totally reflected. Under this condition an ultra thin (around 100 to 200 nm) "evanescent electromagnetic field" of light forms on the side the cells
CD BioSciences' Nikon TIRF/FRAP Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.