Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Widefield Cryo Leica DM6000 FS-based confocal microscopy imaging services.
CLEM cryo-workflow
Cryo-fluorescence acquisition on vitrified samples
1. Designation
Widefield Cryo
2. Model
Leica EM-Cryo-CLEM
3. Objectives
Leica HCX PL APO 50× / 0.90 CLEM
4. Software
Matrix MAPS module in LAS X
5. Filter Cubes
DAPI / GFP / TRITC
6. General Informations
1) Cryo-chamber and cryo-transfer shuttle for cryogenic imaging. For single 3 mm electron microscopy grids and Autoloader™ sample loader from FEI system.
2) Motorized stage in X and Y to acquire mosaics of images
3) Greyscale sCMOS camera Hamamatsu Flash4.0 LT+ for fluorescence
7. Acquisition Modes
Transmission and fluorescence with the greyscale camera in cryogenic conditions
Mosaics of images (scan-slide)
8. Category
Photonic imaging
CD BioSciences' Widefield Cryo Leica DM6000 FS-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.