Microscope Imaging Service—LSM 700

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with LSM 700-based confocal microscopy imaging services.

Name of the Instrument/Platform

LSM 700

Introduction

Technical specifications
1. Wide Field Illumination
1) Halogen lamp 12V, 100W
2) HXP 120W
3) Filter sets DAPI, GFP, Rhodamine
2. laser lines:
solid state laser:
1) 405nm, 5mW
2) 488nm, 10mW
3) 555nm, 10mW
4) 639nm, 5mW
3. Detectors:
1) PMT 1: SP 490, SP 555, SP 640nm
2) PMT 2: LP 490, LP 560, LP 640nm
3) VSD Beamsplitter: 416-728 in 1nm steps
4) Transmitted light: T-PMT
4. Lenses:
5. 5×/ 0.16 plan apochromat
1) 10×/ 0.3 EC Plan-Neofluar
2) 20×/ 0.8 plan apochromat
3) 40×/ 1.3 plan apochromat, oil, DIC
4) 63×/ 1.4 plan apochromat, oil, DIC
6. Software:
1) ZEN 2012 black
2) Physiology package (time series analysis software, ROI analysis, online and offline calibration of ion concentrations)
3) Multiple Time Series
4) Experiment Designer Modules
5) Tile and Stitch Module
6) Tiles and Positions Module
7. Other:
1) XYZ motorized
2) DIC
3) on request 100× lens, 40×/ 1.1 LD C apochromat
4) Water
5) Mic table insert for 4 slides on request

Summary

LSM 700 is an inverted point laser scanning microscope. It is equipped with 4 solid-state lasers. This microscope has a pinhole that is fully automatically closed and opened steplessly and therefore the optimum section thickness is always set for each wavelength in multifluorescence experiments. In addition, the emission light can be continuously distributed to both detectors on the system and therefore offers the possibility of a free choice of the detection area. The availability of a smart setup for the settings makes this device very easy to use.
The system enables the following applications (or combinations thereof):
1. Imaging of fixed samples (tissues, cells)
2. Z stack (3D)
3. Time series
4. Recording from different positions
5. Capturing overview images

CD BioSciences' LSM 700-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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