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Microscope Imaging Service—Leica TCS SP8 STED Microscope

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Leica TCS SP8 STED Microscope-based confocal microscopy imaging services.

Name of the Instrument/Platform

Leica TCS SP8 STED Microscope

MANUFACTURER:
Leica

Introduction

The instrument introduces important advances in three crucial areas: super-resolution in all dimensions (STED in 3D), super-resolution over the whole spectrum and in live cell imaging. To accomplish super resolution in the Z dimension the instrument has a second light path with a different phase mask. The multi-color super resolution is achieved with three laser lines: two continuous wave lasers at 592 nm and 660 nm and a pulsed laser at 775 nm. In addition, the White Light Laser, the AOBS (acousto optical beam splitter) and tunable spectral detectors allows any kind of fluorophore combination. To improve resolution with live cell imaging we acquired Gated STED (allows to shift the time gate away from the excitation pulse), that not only increases resolution but less laser power is needed enhancing the viability of the cells. We are also offering light-sheet imaging capabilities.
In addition to the acquisition software provided by the manufacturer, the STED microscope is also equipped

Summary

The microscope enables mechanistic and morphological studies in biology, biomaterials and tissue engineering due to the instrument's nanoscale resolution combined with its multiple modalities: live-cell imaging; quantitative studies of expression and/or co-localization; 3D imaging of biomolecules and bio-material morphologies; and dynamic imaging. It will also play a crucial role in the novel application of fluorescent dyes and will be instrumental in visualizing different aspects of tissue engineering.

CD BioSciences' Leica TCS SP8 STED Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.
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