Microscope Imaging Service—Lattice Lightsheet Microscope

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Lattice Lightsheet Microscope-based confocal microscopy imaging services.

Name of the Instrument/Platform

Lattice Lightsheet Microscope

Introduction

Sample Preparation
1. This microscope takes 5 mm coverslips (thickness is not important) mounted on the holder shown below.
2. The stage can be moved within a 3 × 3 mm region from the centre of the coverslip
3. Samples must be adhered to the coverslip (for non-single cell imaging please consult with facility staff)
4. Samples are imaged with water dipping lens' with your sample in a 8 mL water bath (take this into account if your require stimulants/drugs in your imaging media)
5. The stage/water-bath is heated, however CO₂ is not available while imaging.
6. The LLSM room has an incubator set to 37℃ 5% CO₂.

Feature Highlights

Highlights
1. Very High spatial resolution (230 nm, 230 nm, 300 nm, xyz)
2. Very High Temporal resolution (100 slices / second)
3. Very low photo-toxicity
4. Opportunity to generate very rare data (very few working systems world wide)
5. 4 Individual lasers (440 nm (CFP), 488 nm (GFP), 560 nm (RFP), 640 nm (Far Red))
Challenges
1. Massive data sets are generated by this system (terabytes)
2. Visualisation and analysis software solutions are not equipped for large volumes of LLSM data, instead MIPS are generated for indexing of files, and finding sections of movies for further analysis. Large collaborations are underway to develop tools to fix this.
3. This system is not a 'turnkey solution' like our Zeiss systems, therefore the software is not as refined, and often many alignments are required to optimise the imaging output.

Summary

The Lattice Lightsheet microscope is an advanced live cell microscope developed by Nobel Prize winner Eric Betzig in 2014. This microscope is extremely gentle on cells allowing for very long acquisitions, as well as being super-fast, (capturing full 3D volumes in under a second).

CD BioSciences' Lattice Lightsheet Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

Related Services