Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon A1-Rsi Confocal-based confocal microscopy imaging services.
The system is mounted on a Nikon Ti-E widefield, inverted, fluorescence microscope with DIC/Phase of 10×, 20×, 40× and 60× dry/oil/water immersion optics. It has 7 laser lines, comprises of diode lasers (for 405 nm, 561 nm and 640 nm) and argon multi laser lines (for 457/477/488/514 nm). Enhanced Spectral Imaging allows fast spectral imaging at 16 fps (512 × 64 pixels) and real-time Spectral Unmixing.
The Nikon A1-Rsi is designed for live cell imaging using Okolab environmental chamber to regulate temperature and air/CO2/N2. It has Nikon's Perfect Focus System (PFS) to maintain focus on sample by compensating for thermal drifts and Prior motorized stage for tiling large areas and multi-point mark and find (high-content imaging) with various stage adapters for different type of petri-dishes, slides and multi-well plates. The system can also be used for Reflectance Confocal Microscopy technique.
Nikon NIS Elements C imaging software allows users to perform real-time
Nikon A1-Rsi is a confocal system equipped with a unique hybrid confocal scan head with 5 highly sensitive PMT (4-fluorescences and 1-transmitted light) detectors and a 32-channel PMT spectral detector for 400-750 nm bandwith. The system allows increased light detection efficiency and has a standard high-resolution confocal galvano mode (up to 4096 × 4096 pixels) as well as a high speed resonant mode capable of scanning 420 fps at 512 × 32 pixels (30fps at 512×512 pixels).
CD BioSciences' Nikon A1-Rsi Confocal-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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