Imaging Service—Olympus FV1200 Upright Confocal Microscope

1. High quality images: Up to 2048 × 2048 pixel resolution and 12-bit gray scale.
2. Scan rotation and region of interest (ROI) scanning: The mounting of an optional AOM (Acousto Optical Modulator) enables free shape scanning. It is effective for bleaching specific areas in FRAP/FLIP experiments or optical stimulation with a 405nm laser.
3. Precision objectives for aberration-free confocal microscopy: CFI Plan Apo VC series objectives correct axial chromatic aberration on the whole visible light spectrum up to 405 nm (h line)
4. Broad selection of laser options: Accommodates a variety of lasers with wavelengths ranging from 405 to 633nm. It also supports solid state lasers.
5. 3D Imaging: Clear, high-density Z-axis images are obtainable. 3D images provide information about the cross section of a specimen.
6. Time-lapse Imaging: This system enables optimum time-lapse imaging for individual experiments, such as the recording of changes in fluorescence intensity in FRET analysis and similar experiments.
7. Fluorescence Recovery After Photobleaching (FRAP) : After bleaching only a specific area, you can measure changes in fluorescence intensity over time to observe the recovery process through changes in fluorescence accompanying cell movement.
8. Multimode Imaging: One microscope supports various imaging modalities, including confocal, TIRF, and epifluorescence, allowing you to make simultaneous observations of cells in the same field of view.
9. Fluorescence Loss in Photobleaching (FLIP) : FLIP allows you to repeatedly bleach the desired area, and then observe how the fluorescence intensity of the unbleached areas is changing.
10. Perfect Focus System (PFS) : This system incorporates an automatic focus maintenance mechanism to continuously correct focus drift caused by temperature changes resulting from reagent droplets or prolonged imaging.