Genomics Service—MassArray System with Epityper DNA Methylation Analysis

Rely on CD BioSciences' experienced genomics services to deliver high-quality, interpretable results using highly sensitive PCR, real-time PCR, NGS technologies, and advanced data analysis. All of your genomics research needs can be met with our highly flexible and customizable platform and our long experience in analyzing preclinical research samples.

Name of the Instrument/Platform

MassArray System with Epityper DNA Methylation Analysis

Introduction

EpiTYPER biochemistry starts with bisulfite treatment of genomic DNA, followed by PCR amplification of target regions. The reverse primers contain a T7 promoter tag. Next, in vitro RNA transcription is performed, followed by base-specific RNA cleavage. Finally, the cleavage products are analyzed using MALDI-TOF mass spectrometry (MassARRAY Analyzer). The methylated and non-methylated cytosine residues in the original genomic DNA are easily distinguished using EpiTYPER Software.
Available Formats
• 96 well Chips

Feature Highlights

• Interrogates 10s-100s of samples and CpG sites in amplicons from 200 - 600 bp and detect down to 5% differences in methylation.
• The EpiTYPER technology includes online assay design software, robust biochemistry steps, and precise detection of reaction products using highly quantitative mass spectrometry.
• Can also be used for other nucleic acid analysis methods, including SNP and somatic mutation detection.

Applications

• Targeted DNA methylation
• Genotyping and mutation detection

Summary

The MassArray System is a complete system for discriminating methylated vs. non-methylated DNA; includes software, reagents, and MALDI-TOF mass spectrometry for detection and quantification.

CD BioSciences offers you MassArray System with Epityper DNA Methylation Analysis-based genomic services. If you are interested, please feel free to contact us for more information.

For research use only, not intended for any clinical use.

Related Services