Genomics Service—CRISPR Platform

Rely on CD BioSciences' experienced genomics services to deliver high-quality, interpretable results using highly sensitive PCR, real-time PCR, NGS technologies, and advanced data analysis. All of your genomics research needs can be met with our highly flexible and customizable platform and our long experience in analyzing preclinical research samples.

Name of the Instrument/Platform

CRISPR Platform

Introduction

1. Synthetic CRISPR Platform
The synthetic CRISPR platform enables gene knockout in an arrayed format using liquid handling automation in 96- or 384-well format. This platform uses synthetic purified sgRNAs that are transiently delivered into Cas9-stable cells using lipid transfection reagents or nucleofected with purified Cas9 protein. The phenotype is assessed typically within 72-96 hours. This platform enables cell phenotyping approaches using high content imaging, fluorescence/luminescence plate reader end points or FACS profiling.
1) Resources available:
• Human whole genome arrayed sgRNA library (Horizon Discovery)
a. Screen as a whole genome (61 plates) or cherry pick custom sub-sets
• Arrayed synthetic single crRNA kinome
• Cas9 virus or protein
• Nucleofection reagents
• Purchasing sgRNAs at discounted rates
2. Viral CRISPR Platform
We house both pooled and arrayed viral CRISPR libraries. Pooled CRISPR library are analysed using next generation sequencing. We take you through

Summary

CRISPR screens enable researchers to determine resistance in their cellular model, facilitate biomarker discovery in patient samples or determine genes responsible for synthetic lethality. We supports both synthetic and pooled CRISPR screening strategies.

CD BioSciences offers you CRISPR Platform-based genomic services. If you are interested, please feel free to contact us for more information.

For research use only, not intended for any clinical use.

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