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With CD BioSciences fluorescence imaging services, we can meet the demands of any biological research requiring wide-field, confocal, and TIRF fluorescence microscopy. Our mission is to support research by advising and optimising the acquisition, analysis and presentation of imaging data. We offer you a OPT Bioptonics 3001-based fluorescence imaging service.
OPT is a 3D imaging technology for biological specimens up to 1×1,5cm in size.
It is a convenient method for viewing the 3D anatomy, gene and protein expression patterns during embryo development, or separate organs and biopsies.
We are using Bioptonics 3001 device with the resolution 1024×1024 (~3,1micron) which allows analyzing specimens in 4 channels:
1. Bright field: in situ hybridization specimens (BMP purple, NBT/BCIP), beta-galactosidase and other stains;
2. GFP1 425/40 nm exciter, 475 nm LP emitter: auto-fluorescence and general morphology;
3. GFP 470/40 nm exciter, 515 nm LP emitter;
4. Texas red 560/40 nm exciter, 610 nm LP emitter.
The raw data obtained from scanner is further processed and analyzed by the 3D analysis software. TIC has license for the Bitplane Imaris software that allows data visualization and detailed morphometric studies of the OPT specimen.
1. Tissue staining for the OPT (immuno-, beta-gal ect.)
2. Sample preparation for OPT: embedding and clearing
3. Scanning and reconstruction
4. Data analysis (Quantification, 3D shape measurements, 3D and 2D linear measurements)
5. Recovery of the specimens for the following use (histology analysis, IHC & ect.)
If you are interested in this service, please feel free to contact us for more information.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.