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Cryo-electron microscopy (CryoEM) applies cryo-fixation technology to observe samples with a transmission electron microscope at low temperatures, to obtain the microscopic technology of the structure of biological macromolecules. After the piece is frozen and placed in the electron microscope, the electrons are scattered through the sample and the nearby ice layer. Use the detector and lens system to image and record the scattered signal, then process the signal, and finally use the three-dimensional reconstruction technology to obtain the three-dimensional structure of the sample. CryoEM imaging services are provided by CD BioSciences based on Titan Krios Electron Microscope.
This state of the art electron microscope is located in a special, dehumidified room, with the microscope sitting on a piezoelectric anti-vibration table. It can be operated at either 200 or 300kV of accelerating voltages, and it is equipped with an X-FEG, which is a highly coherent Schottki type electron emitter. The images are collected on a Gatan K3 direct electron detector, after passing through a BioQuantum energy filter. Data can be collected using native ThermoScientific software for image acquisition (EPU and Tomo), or SerialEM.
CD BioSciences offers its customers the CryoEM imaging service based on Titan Krios Electron Microscope to help analyze the 3D structure and microstructure of samples. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.