Confocal Microscope Imaging Service—Olympus LEXT OLS5000 Profilometer

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Olympus LEXT OLS5000 Profilometer-based confocal microscopy imaging services.

Name of the Instrument/Platform

Olympus LEXT OLS5000 Profilometer

Introduction

The Olympus LEXT OLS5000 is a laser confocal microscope designed specifically to measure shape and surface roughness of samples at the submicron level.
It is a complementary tool to that of AFM which is traditionally and predominantly used for high resolution height and surface roughness measurements of much smaller areas. The instrument utilises a 405nm violet laser and a pinhole aperture much the same as traditional confocal microscopes, with the addition of a MEMS resonant scanner used for XY scanning of the UV laser for low distortion and aberrations.

Required Training

When measuring height of the sample, the microscope acquires multiple confocal images through a Z range defined by the user. Based on focus position (Z) and detected light intensity, light intensity variation curves are created for each pixel. Following this a height information/image can be reconstructed, along with an "extended" image which is an image of the sample surface in which the entire surface is in focus.
1. Sample sizes up to 100 mm high
2. 100 mm × 100 mm motorised stage
3. 5×, 10×, 20×, 50× LEXT normal WD objectives, 20×, 100× long WD objectives
4. DIC option
5. 4k × 4k resolution of scanned images
6. CMOS colour camera for optical image
7. Height/Width Accuracy: measurement value +/- 1.5%
8. For full range of specifications click here

Important Notes

Samples:
Providing sample can fit onto stage and doesn't exceed weight limit (2-3 kg), the full range of samples including but not limited to biological, metallurgical, and geological can be analysed.

CD BioSciences' Olympus LEXT OLS5000 Profilometer-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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