Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon A1R Laser Scanning Confocal with DS-Ri1 CCD Camera-based confocal microscopy imaging services.
The Nikon A1R laser scanning confocal is a research grade confocal microscope capable of high resolution imaging.
It has three solid state lasers (405 nm, 561 nm and 640 nm) and a multi-line Argon gas laser (457 nm, 488 nm, 514 nm).
The unique hybrid scan head has a galvano scanner for high resolution imaging and a resonant scanner for high speed acquisition. The resonant scanner allows high-speed imaging ranging from 30 fps (512 × 512 pixels) to 420 fps (512 × 32 pixels), and the galvano scanner features a maximum scan rate of 4 frames per second at 512 × 512 pixels. By employing a hexagonal pinhole, higher brightness than that of a circular pinhole is achieved.
The A1R confocal has two detectors. The "standard" 4 channel hybrid detector has two GaAsP PMTs and two normal PMTs. The spectral detector has wavelength resolutions of 2.5, 6, and 10 nanometers, and a 32-channel multi-anode photomultiplier. Spectral unmixing allows separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Real time unmixing can be applied during image acquisition.
The Ni-E motorized microscope is sited on TMC vibration isolation table. The microscope has two dry objectives (10× Plan Apochromat Lambda; 20× Plan Apochromat Lambda), two water immersion objectives (40× Apo LWD WI λS; 60× Plan Apo VC WI) and one oil immersion objective (100× Plan Apochromat Lambda H Oil, N.A.1.45, W.D.0.13mm). Fluorescence filters are longpass: V-2A (Ex 330-380, DM 400 BA 420) ; UV-2A (Ex 380-420, DM 430 BA 450) ; B-2A (Ex 450-490, DM 505 BA 520) ; G-2A (Ex 510-560, DM 575 BA 590).
The microscope has a zooming port and DS-Ri1 colour cooled digital ca
CD BioSciences' Nikon A1R Laser Scanning Confocal with DS-Ri1 CCD Camera-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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