Confocal Microscope Imaging Service—Nikon A1R Confocal Microscope

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon A1R Confocal Microscope-based confocal microscopy imaging services.

Name of the Instrument/Platform

Nikon A1R Confocal Microscope

MANUFACTURER:

Nikon

Introduction

For general imaging, the A1R offers three operating modes. In the standard mode the signal of the fluorochromes that are excited by six available lasers (405, 445, 488, 514, 561 and 640 nm) is separated by dichroic mirrors and optical filters. Up to four channels can be acquired in a line mode using two normal and two ultra sensitive GaAsP-detectors. If fluorochromes are used whose emission is too close to be separated by this method (like GFP and YFP) , a spectral detector does the job: here the fluorochrome emission can be split up in up to 32 bands of 10 nm bandwidth, detected by a GaAsP-detector. By this these signals can be clearly and reliably distinguished by a process called "spectral unmixing" implemented in the NIS-Elements image acquisition and analysis software. Spectral imaging also makes it possible to separate a fluorescent background from specific signal.
The third mode is called "virtual filters". Here the spectral detector is used to freely define ranges where signal

Summary

The A1R features a so called "hybrid scanner", that is two scanners in one scanning-head: a glavano scanner for low-noise images ranging in resolution from 64 × 64 up to 4096 × 4096 pixels and a resonant scanner that is capable of taking up to 30 full 512 × 512 pixel frames per second. What is more, both scanners can be used in parallel: while the fast resonant scanner is acquiring images the galvano scanner may perform bleaching or activation of user defined regions of interest.

CD BioSciences' Nikon A1R Confocal Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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