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The CD BioSciences biophysical characterisation laboratories are equipped with state-of-the-art instruments for studying proteins, nucleic acids, lipids, and complexes. Researchers can benefit from our Nanotemper Monolith Thermophoresis Instruments-based biophysical characterisation services.
Microscale Thermophoresis (MST) is a powerful method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size.
In an MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins or intrinsic tryptophan fluorescence.
The applications range from small-molecule binding events to protein-protein interactions and interactions of multi-protein complexes.
• access affinities (Kd, dissociation constant) : pM/nM to mM range
• examine: proteins, peptides, ribosomes, ions, nucleosomes, liposomes and more
• study membrane proteins directly in liposomes or detergent solution
• study multi-component reactions such as ternary complex formation, order of assembly, interfering factors, cooperativity
• measure in any buffer, including complex detergent mixtures
• examine interactions under physiological assay conditions such as serum, cell lysate
• study stoichiometry and discriminate between different binding sites on a target
• study the binding energetics ΔG (free energy), ΔH (enthalpy) and ΔS (entropy)
Microscale Thermophoresis (MST) is a powerful method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size and translates this into a measure of binding affinity.
In the chemistry-biology community, our service is one of the best options for teams working on biophysical characterisation. Do not hesitate to contact us if you are interested in this.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.