Biophysical Characterization Service—Jasco J-1500 Circular Dichroism Spectrophotometer

The CD BioSciences biophysical characterisation laboratories are equipped with state-of-the-art instruments for studying proteins, nucleic acids, lipids, and complexes. Researchers can benefit from our Jasco J-1500 Circular Dichroism Spectrophotometer-based biophysical characterisation services.

Name of the Instrument/Platform

Jasco J-1500 Circular Dichroism Spectrophotometer

Introduction

Circular dichroism (CD) spectroscopy measures differences in the absorption of left-handed versus right-handed polarized light that result from structural asymmetry.
This measured difference gives important data regarding the structure and conformation of the molecule of interest. Any functional group that absorbs light will have a distinct CD signature within each absorption band, making CD spectroscopy useful in investigating the structures of small organic molecules, nucleic acids, higher-weight proteins, polymers, and polypeptides.
The secondary and tertiary structural components of the molecule of interest will impart a CD signature in the UV spectrum. This technique can provide an indication of the percentage of the molecule that is in each conformation (α-helix, β-sheet, β-turn, etc.), which can then be used in tandem with other techniques to deduce the complete structure. Using these principles, denaturation and other conformational changes, along with the thermodynamics of s

Summary

Circular dichroism (CD) spectroscopy is a powerful technique to probe the secondary and tertiary structures of proteins and peptides. The Jasco J-1500 and the accessories allow researchers to run a wide range of experiments.

In the chemistry-biology community, our service is one of the best options for teams working on biophysical characterisation. Do not hesitate to contact us if you are interested in this.

For research use only, not intended for any clinical use.

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