Biophysical Characterization Service—Analytical Ultracentrifuge Fluorescence Detection System (AU-FDS)

The CD BioSciences biophysical characterisation laboratories are equipped with state-of-the-art instruments for studying proteins, nucleic acids, lipids, and complexes. Researchers can benefit from our Analytical Ultracentrifuge Fluorescence Detection System (AU-FDS)-based biophysical characterisation services.

Name of the Instrument/Platform

Analytical Ultracentrifuge Fluorescence Detection System (AU-FDS)

Introduction

Features:
• High Sensitivity (picomolar sample concentrations)
• Large Dynamic Range (approximately four decades)
• High Selectivity (measures only fluorescing molecules)
• Higher Throughput (both sample and reference channels can be loaded with sample)
The FDS works as an add-on to existing Beckman analytical ultracentrifuges, old or new. The original Beckman instrument operation is not affected. The AU-FDS utilizes confocal optics to track the location of fluorophores. High-speed electronics measure the entire rotor rotation, allowing all samples to be measured simultaneously.

Applications

• Higher sensitivity and large dynamic range allow determination of tighter binding constants.
• Very high selectivity due to selection of excitation wavelength (488 nm) allows studies with complex mixtures that traditional detectors cannot handle: heterogeneous binding of proteins with the same molecular weight; serum, cell lysates, et cetera.

Summary

The Analytical Ultracentrifuge Fluorescence Detection System (AU-FDS) accessory for the Beckman XL-A / XL-I instruments compliments the existing detectors.

In the chemistry-biology community, our service is one of the best options for teams working on biophysical characterisation. Do not hesitate to contact us if you are interested in this.

For research use only, not intended for any clinical use.

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