Biological Imaging Service—Zeiss Celldiscoverer7

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Zeiss Celldiscoverer7-based bioimaging services.

Name of the Instrument/Platform

Zeiss Celldiscoverer7

MANUFACTURER:

Zeiss

Introduction

Objectives:
1. PlanApochromat 5×/0.12
2. PlanApochromat 20×/0.7 and 20×/0.95, Autocorr
3. PlanApochromat 50×/1.2 water, Autocorr and autoimmersion
4. These objectives are used in combination with a 0.5×, 1× and 2× optovar lens, which permits 2.5×, 5×, 10×, 20×, 25×, 40×, 50×, 100× magnifications.
Laser Lines:
405 nm, 488 nm, 561 nm, and 640 nm
Detectors:
1. For LSM900 Confocal:
1) Airyscan 2 detector
2) 2 PMT detectors
2. For Wide-field Imaging:
1) Axiocam 506 black and white camera (2752 × 2208 pixels)
Stage Inserts:
The system accommodates most multiwell plates, 3.5 and 6 cm petri dishes, microscopy slides, and chamber slides
Operating Software: Zen Blue

Applications

1. Live cell imaging
2. High-throughput imaging
3. Tiling
4. Time-lapse
5. Optical sectioning for 3D imaging
6. Super resolution imaging with the Airyscan 2
7. FRAP and FRET

Summary

The Celldiscoverer 7 is an automated boxed microscope with a user-friendly interface that allows wide-field, confocal (LSM 900), and super-resolution imaging by means of its Airyscan2 detector. The system is an inverted microscope capable of live cell imaging experiments with a variety of incubation conditions. It has enhanced automated functionality such as Autocorr objectives, which enable the system to automatically detect a variety of sample holders and adjust for spherical aberrations. The CD7 is also equipped with an autoimmersion system that automatically applies water to its water immersion lenses and maintains the volume of immersion water to permit live cell imaging over a long duration. The system uses LED illumination, which has low phototoxicity and long-term stability.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

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