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Biological Imaging Service—Leica SP5 Confocal Microscope

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Leica SP5 Confocal Microscope-based bioimaging services.

Name of the Instrument/Platform

Leica SP5 Confocal Microscope

MANUFACTURER:
Leica

Introduction

1. Stand: inverted, Leica DMI6000
2. Stage: motorized stage for 3D stack, tiling, and multipoint image acquisition; sample holder will accommodate slides and small (60 mm) petri dishes
3. Fluorescent filters available: for direct observation through eyepieces GFP, Texas Red, and simultaneous GFP + Texas Red; filters for confocal operation are spectral (ATOF)
4. Transmitted light modes: bright-field, DIC, and polarized light.
5. Laser lines: 405, Argon (458, 476, 488, 496, and 514) and HeNe (543, 594, and 633)
6. Objectives:
1) 10×/0.3 NA (wd-11 mm)
2) 20×/0.75 NA (wd-0.59 mm)
3) 40×/1.25-0.75 NA oil DIC (wd-0.1 mm)
4) 63×/1.4-0.6 NA oil DIC (wd-0.1 mm)
5) 63×/1.2 NA water (wd-0.14 mm)
7. Software: Leica Application Suite Advanced Fluorescence
8. Detectors: 3 channels available for simultaneous fluorescent image acquisition, 1 available for transmitted light image acquisition; standard scanning and fast resonance scanning (8000 Hz) modes available.

Summary

Best suited for thick (10-150 microns) samples mounted on a standard microscope slide with a coverslip or in a small petri dish. Used for FRAP, photo-activation, and scanning irregular regions of interest. Non-labeled and fluorescently-labeled samples may be used. Can capture highly dynamic processes if operated in resonance scanning mode.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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