Biological Imaging Service—Solamere Spinning Disk Confocal Microscope

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Solamere Spinning Disk Confocal Microscope-based bioimaging services.

Name of the Instrument/Platform

Solamere Spinning Disk Confocal Microscope

Introduction

1. Stand: inverted, Nikon TE2000
2. Stage: motorized stage for 3D stack and multipoint image acquisition; sample holder available to accept slides, petri-dishes, and multi-well plates
3. Fluorescent filters available:
—for direct observation through eyepieces:
1) DAPI: EX BP 360/40 : DC 400 : EM BP 460/50
2) GFP: EX BP 470/40 : DC 495 : EM BP 525/50
3) TRITC: EX BP 540.5/25 : DC 565 : EM BP 620/60
—for confocal operation:
4) DAPI: EM 460/50
5) GFP+RFP: EM BP 528/80
6) GFP: EM BP 525/50
7) RFP: EM BP 593/40
8) Cy5: EM BP 700/75
4. Transmitted light modes: bright-field and DIC
5. Laser lines: solid state 405, 488, 561, 640
6. Objectives:
1) 10×/0.3 NA (wd-16 mm)
2) 20×/0.50 NA (DIC) (wd-not available)
3) 40×/0.75 NA (wd-0.66 mm)
4) 40×/1.3 NA oil (wd-0.2 mm)
5) 60×/1.4 NA oil (DIC) (wd-0.13 mm)
6) 100×/1.4 NA oil (DIC) (wd-not available)
7. Software: Micro-Manager
8. Scanner: Yokogawa CSU-X1 spinning disk
9. Camera: Hamamatsu ImagEM X2

Summary

Best suited for imaging of thick (10-150 microns) live samples mounted on a standard microscope slide with a coverslip, in a small dish, or in an incubation chamber. Non-labeled and fluorescently-labeled samples may be used.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

Related Services