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Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Andor Dragonfly-Confocal/TIRF/Super Resolution-based bioimaging services.
1. Objectives: 10×/0.45, 20×/0.8, 40×/1.1 W, 40×/1.3 Oil, 63×/1.47 Oil.
2. Laser lines: 405 nm, 488 nm, 561 nm, 638 nm, 735 nm.
3. Detectors: Two Andor Zyla 4.2 sCMOS cameras can be used together to acquire two colors simultaneously.
4. Operating software: Fusion
1. Spinning disk confocal imaging. In contrast to point scanning confocals, such as the LSM880 and CD7 that serially acquire one focal point at a time to reconstitute a 2D image, the Dragonfly acquires confocal images by simultaneously acquiring thousands of spots from the focal plane. This setup enables the Dragonfly to provide several important advantages over point-scanning confocals:
1) Faster speed (up to ~200 frames per second), which is ideal for fast 2D or 3D time-series or tile scanning.
2) Gentler imaging - photobleaching and phototoxicity are greatly reduced, which is better for live cell imaging.
3) The high sensitivity of cameras is well suited for dim specimens.
2. Super-resolution imaging. The Dragonfly can perform 3D localization- based microscopy techniques (dSTORM, PAINT, etc.) that improve spatial resolution to ~30 nm laterally, and ~50 nm axially.
3. TIRF microscopy. a technique allowing the confined excitation of fluorophores within ~100 nm above the coverslip.
The Dragonfly microscope is a fully automated multimodal microscope that can be used for confocal, total internal reflection microscopy (TIRF) and super-resolution (localization-based techniques such as: PALM, dSTORM, PAINT, etc.).
In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.